File created in the Standard Flowgram Format (SFF), a binary file format used for DNA sequencing; saves the output from a 454 Life Sciences sequencing machine (e.g., a GS Junior or GS FLX System), which takes an input DNA sample and generates the DNA sequence.
Example programs that can open and edit SFF files include SFF Workbench (formerly 454 SFF Tools) and MATLAB. If using MATLAB, you should use the sffread() function, which returns the SFF data in a MATLAB array of structures. 454 Life Sciences also provides a toolkit for processing and analyzing SFF files, called the GS Data Analysis Software.
Graphics file used by M.U.G.E.N., a freeware 2D fighting game engine; stores sprites, which are graphics that store the animation states for a fighter or other animated object; can store sprites for jumping, kicking, punching, running, or other movements.
SFF files are created from .PCX bitmap image using the sprmaker program included with the M.U.G.E.N. game engine toolsuite.